Role of Central Nervous System RyR1 Calcium Channel Functions in Neurodegenerative Diseases and Malignant Hyperthermia

Objectifs

This project aims to determine the role of RyR1 in central nervous system function and to assess the impact of malignant hyperthermia-associated mutations on neuronal activity. It will investigate the consequences of RYR1 deletion or mutation on thermoregulation, brain connectivity, and calcium signaling.

Résumé

The ryanodine receptor type 1 (RyR1) is a key intracellular calcium channel essential for skeletal muscle function, but it is also expressed in specific regions of the central nervous system, where its role remains poorly understood. Emerging evidence suggests that RyR1 contributes to neuronal calcium signaling and may be involved in the pathophysiology of malignant hyperthermia and neurodegenerative diseases. However, the impact of RyR1 dysfunction in the brain, as well as the consequences of disease-associated mutations, is still largely unknown. This project aims to clarify the role of RyR1 in central nervous system function and to determine how its alteration affects brain physiology, with a particular focus on thermoregulation, neural circuit integrity, and disease mechanisms. Ultimately, it seeks to establish RyR1 as a key player linking calcium homeostasis to neurological disorders.

Méthodes

The project combines conditional mouse models, viral-mediated gene deletion, and knock-in models to investigate the role of RyR1 in the brain. Brain structure and connectivity will be assessed using anatomical and diffusion MRI, while neuronal calcium signaling and synaptic function will be analyzed by two-photon calcium imaging and electrophysiology. Behavioral, molecular, and in vivo rescue approaches will further evaluate the impact of RyR1 dysfunction and disease-associated mutations.
This project is co-led by J. Fauré, a geneticist and muscle specialist, F. Lanté, an electrophysiologist, and J.-C. Deloulme, a neuroanatomist..

Références

Deloulme at al. Structural interhemispheric connectivity defects in mouse models of BBSOAS: Insights from high spatial resolution 3D white matter tractography. Neurobiol Dis. 2024.

Maino A, et al. Identification of a novel de novo RYR1 variant associated with malignant hyperthermia : A case report. Eur J Anaesthesiol. 2026

Rolland M et al. Effect of Aβ Oligomers on Neuronal APP Triggers a Vicious Cycle Leading to the Propagation of Synaptic Plasticity Alterations to Healthy Neurons

Domaines d'expertise requis

Neuronal calcium signaling ; Brain MRI ; Synaptic plasticity ; Mouse models of neurological diseases

Contact

Jean-Christophe Deloulme, CRHC Inserm
Email: jean-christophe.deloulmeinserm.fr (jean-christophe[dot]deloulme[at]inserm[dot]fr)