FLORENCE FAUVELLE

I am specialized in Nuclear Magnetic Resonance (NMR) spectroscopy of small molecules, mainly in biomedical research field.

► Metabolomics to investigate metabolism and to improve diagnosis

• HRMAS NMR (High Resolution Magic Angle Spinning) for unprocessed sample analysis,
• High resolution NMR  (biofluids e.g. serum, cerebrospinal fluid, fecal water...)
• Multivariate statistics : PCA, OPLS-DA..

► NMR study of interactions

• Intercalation of heterocycles with synthetic oligonucleotides
• Complexation of small molecules with different  cyclodextrins, using multi-nuclei NMR and mass spectrometry (thesis)
• Drugs-membranes interactions (phospholipidic Liposomes) using  ¹H, ³¹P, ²H NMR.

► Quantification of NMR signals

• Semi-automatic quantification of HRMAS NMR brain tissus, collaboration to the development of  jMRUI/QUEST algorithm (collaboration D. Graveron, Université Lyon1, La Doua, Villeurbanne).

► Master 2, UFR Chimie biologie, UGA

• Master 2 “High throughput in biology”, “High throughput NMR: metabolomics” 3h/year since 2015.
• Master 2 “Cancer disease: experimental and therapeutic approaches”, “Metabolomics in cancer research” 3h/year, 2015-2023.

► AI4OneHealth, UGA

• AI4 omics, “NMR-based metabolomics”, 2021-2023.

► DUT Mesures Physiques, UGA

• Initiation to NMR, 8h/year, 1999 to 2009,
• Sectroscopy, 45h/year, 1999 to 2001.

Education:

► Animal experimentation for manager (level I), University Grenoble Alpes (2011).

► Habilitation à Diriger les Recherches (HDR) University Grenoble Alpes (2010)  "Application of HRMAS NMR to metabolism and metabolomics studies"

► PhD University Grenoble Alpes, (1999) very honorable mention with congratulations, Biological and medical engineering. " Biologic decontamination of toxic cations: the cyclodextrin solution"

Positions

► Grenoble Institut of neurosciences since 2013, "Functionnal Neuroimagering and brain perfusion"

 IRMaGe facility, R&D in NMR-based metabolomics since 2013.

► Ministry of Defence Biomedical research center  (CRSSA), 1990-2013, "Cellular and molecular biophysics" NMR team, R&D in NMR-based metabolomic

Director of NMR facility

Most representative publications

Mallet D., Goutaudier R., Dufourd T., Barbier EL., Carnicella S., Colca JR., Fauvelle F.* Boulet S.* (* Co-senior authors). Re-routing metabolism by the mitochondrial pyruvate carrier inhibitor MSDC-0160 attenuates neurodegeneration in a rat model of Parkinson’s disease, Molecular Neurobiology, DOI: 10.1007/s12035-022-02962-9, 2022.

Mallet D., Dufourd T., Decourt M., Carcenac C., Bossù P., Verlin L., Fernagut PO., Benoit-Marand M., Spalletta G., Barbier EL., Carnicella S., Sgambato V., Fauvelle F.* Boulet S.* (* Co-senior authors). A metabolic biomarker predict Parkinson’s disease at early stages in patients and in animal models, Journal of Clinical Investigations DOI: 10.1172/JCI146400, 2022.

Guillon A. Diakite D., Cezard A., Baranek T., Bourgeais J., Picou F., Hervé V., Carballido J., Nadal Desbarats L., Auvet A., Dingli  F., Turtoi A., Le Gouellec A., Fauvelle F., Crépin T., Hiemstra P.S., Paget C., Loew D., Herault O., Naffakh N., Le Goffic R. and Si-Tahar M. Host succinate inhibits influenza virus infection through succinylation and nuclear retention of the viral nucleoprotein, EMBO DOI: 10.15252/embj.2021108306, 2022.

Clement A., Doyen M., Fauvelle F., Hossu G., Chen B., Barbery-Heyob M., Hirtz A., Lamiral Z., Pouget C., Gauchotte G., Karcher G., Beaumont M., Verger A., Lemasson B. In vivo characterization of physiological and metabolic changes related to isocitrate dehydrogenase 1-mutation expression by multiparametric magnetic resonance imaging and spectroscopy in rat model with orthotopically-grafted human-derived glioblastoma cell lines. NMR biomed doi.org/10.1002/nbm.4490, 2021.

Lo Presti C., Fauvelle F., Jacob MC., Mondet J., Mossuz P. The metabolic reprogramming in acute myeloid leukemia patients depends on their genotype and is a prognostic marker, Blood Advances doi.org/10.1182/bloodadvances.2020002981, 2021.

Hamelin S., Stupar V., Maziere L., Guo J., Labriji W., Liu C., Bretagnolle L., Parrot S., Barbier EL., Depaulis A., Fauvelle F. In vivo GABA increase as a biomarker of the epileptogenic zone : an unbiased metabolomics approach, Epilepsia doi.org/10.1111/epi.16768, 2020.

Vial J., Huchedé P., Fagault S., Basset F., Rossi M., Geoffray J., Soldati H., Bisaccia J., Elsensohn M.H., Creveaux M., Neves D., Blay J.Y., Fauvelle F., Bouquet F., Streichenberger N., Corradini N., Bergeron C., Maucort-boulch D., Castets P., CARRE M., Weber K., CASTETS, M. Low expression of ANT1 confers oncogenic properties to rhabdomyosarcoma tumor cells by modulating metabolism and death pathways. Cell Death Discovery doi.org/10.1038/s41420-020-00302-1, 2020.

Lo presti C., Fauvelle F., Mondet J., Mossuz P. The differential activation of metabolic pathways in leukemic cells depending on their genotype and micro‑environmental stress. Metabolomics, doi.org/10.1007/s11306-020-1633-z, 2020

Sinniger V., Pellissier S., Fauvelle F., Trocmé C., Hoffmann D., Vercueil L., Cracowski J.L., David O. A 12-month pilot study outcomes of vagus nerve stimulation in Crohn’s disease. Neurogastroenterology & Motility., doi.org/10.1111/nmo, 2020