Biological techniques platform

Used techniques

The techniques carried out on this technical platform are in various fields:

• Molecular biology: PCR, qPCR, RT-qPCR, Western Blot, Dot Blot, immunofluorescence, immunoprecipitation, protein assays, preparation of protein extracts

• Cellular biology: line maintenance, cell freezing, cell thawing, transfection

• Bacteriology: transformation, isolation, cultivation, induction, preparation of plasmids, purification of proteins

• Histology: Staining of slides, tissue sectioning

• Miscellaneous: PCR analysis, Western Blot quantification, recording results, formatting results, drafting procedures, stock management

• Health and safety: good laboratory practice, chemical waste management, writing procedures, ...

• Training: Training in the various techniques

 

Used equipements

- Fume hood
- Safe weighing station
- Microbiological safety station
- Thermal-cycler
- Electrophoresis system
- UV transilluminator
- Incubator
- Spectrophotometer
- Trans-blot
- Chemidoc
- Microscope
- CO2 incubator
- Cryostat
- pH meter
- Centrifuge
- Vortex
- Magnetic stirrers
- Nitrogen
- Autoclave