MAP6d1 expression characterization in the brain

Objectifs

Precise MAP6d1 localization at different levels: 1/ in different brain region – 2/ subcellular localization in neurons and oligodendrocytes – 2/ in organization of neuronal primary cilia

Résumé

Neurons rely on the microtubule cytoskeleton to establish and maintain their highly polarized architecture while adapting their connectivity and responsiveness to incoming signals. This regulation is partly mediated by microtubule-associated proteins such as MAP6d1. MAP6d1 is expressed postnatally in the brain and acts as a major microtubule stabilizer ; we have recently shown that it regulates microtubule doublet assembly in neuronal primary cilia. Our ongoing work reveals that MAP6d1-knockout mice exhibit altered emotional behavior, a phenotype potentially linked to MAPd1’s role in serotonin signaling via its interaction with 5HT2B receptors. The next critical step in deciphering MAP6d1’s neuronal functions is to precisely map its localization in the brain at different developmental stages and assess its subcellular distribution in neuronal compartments. To achieve this goal, we generated MAP6d1-knockin GFP mice, in which the endogenous MAP6d1 is fused to a fluorescent GFP tag, allowing direct in vivo visualization of its expression. The objective of the Master 2 internship will be to characterize this new mouse line by histological analysis of brain sections and cell biology studies using primary cultures of hippocampal neurons.

Méthodes

Werstern blot, histology (cryostat section and immunofluorescence on floating slices), cell biology (primary neuronal culture dans immunofluorescence), confocal microcopy, expansion microscopy

Référence

Gopal D., Wu J., et al, Nat. Commun. 2025
Boulan B., Ravanello C., et al., ELife 2021
Gory-Fauré et al., Methods Mol Biol 2022

Domaines d'expertise requis

Cell biology and/or histology

Contact

Sylvie Goré-Faure
Email :  sylvie.gory-faureuniv-grenoble-alpes.fr (sylvie[dot]gory-faure[at]univ-grenoble-alpes[dot]fr)