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Grenoble Institut des Neurosciences Grenoble Institut des Neurosciences

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Neuronal activity and the regulation of intra-axonal dynamics and synapse function

Objectifs

The objectives of the project are to understand how neuronal activity regulates intra-axonal dynamics and synapse function using microfluidics systems connected to multielectrode arrays.

Résumé

Dynamic remodeling of axonal connections in the adult brain is a prerequisite for behavioral adaptation to environmental changes. BDNF, the most abundant neurotrophin in the adult brain plays a central role in axonal remodeling that conditions complex cognitive function and long-term memory. But how is BDNF transport in axons regulated and how is it selectively targeted to activated synapses?

Using microfluidics reconstruction of neuronal networks, multielectrode arrays, super-resolution videomicroscopy and computational modeling, we want to investigate modes of transport and decipher the signaling pathways to adapt to high neuronal demands. The goal of this project is to use this model to identify the molecular and cellular events that decode neuronal activity into BDNF transport to the active synapse in health and disease such as Huntington’s disease.

Méthodes

Techniques used will include molecular biology, biochemistry, primary cultures, state of the art live-imaging microscopy and the development and use of new microfluidic devices coupled to micro-electrode arrays to study intracellular dynamics in connected neuronal networks.

Références

  • Virlogeux A et al (2018) Reconstituting Corticostriatal Network On-a-Chip Reveals the Contribution of the Presynaptic Compartment to Huntington’s Disease. Cell Reports, Jan 2;22(1):110-122. doi: 10.1016/j.celrep.2017.12.013
  • Hinckelmann MV, et al., (2016) Self-propelling vesicles define glycolysis as the minimal energy machinery for neuronal transport Nature Communications, 7:13233. doi: 10.1038/ncomms13233
  • Zala D, et al. (2013) Vesicular glycolysis provides on-board energy for axonal transport. Cell. 152, 479-91. doi: 10.1016/j.cell.2012.12.029

Domaines d'expertise requis

Cell biology, neurobiology, imaging techniques, microscopy, microfluidics

Contacts

Frédéric Saudou, UGA Professor, and Maxime Cazorla, Researcher
Email : frederic.saudou@univ-grenoble-alpes.fr

Mise à jour le 31 mai 2018

Contacts

Pour une candidature spontanée, envoyez un email à gincomm[at]univ-grenoble-alpes.fr ou utilisez le formulaire de contact.

Pour les stages de licence et de 3ème, voir les modalités de demande spécifiques sur les pages correspondantes. 

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