Contenu

x

Moteur de recherche interne

Grenoble Institut des Neurosciences Grenoble Institut des Neurosciences

  • Youtube
  • Linkedin
  • Twitter

Accueil > Nous rejoindre > Offres de stages

Accéder au plan complet du site

Energy metabolism as a regulator of axonal transport in health and Huntington’s disease

Objectifs

The objectives of the project are to understand the role of the huntingtin protein in the regulation of intracellular dynamics in neurons and its relation to energy metabolism.

Résumé

Huntington’s disease (HD) is caused by the abnormal polyglutamine expansion in the N-ter part of huntingtin (HTT), a large protein of 350kDa. Over the past years, we proposed that HTT acts a scaffold for the molecular motors and through this function, regulates the efficiency of vesicular transport along microtubules in neurons. Huntingtin also scaffolds glycolytic enzymes that provide energy for axonal transport. Here we propose to study the sources of energy for axonal transport in response to neuronal activity and the role of HTT in this mechanism in both normal and Huntington’s disease conditions.

Méthodes

Techniques used will include molecular biology, biochemistry, primary cultures, state of the art live-imaging microscopy and the development and use of new microfluidic devices to study intracellular dynamics in connected neuronal networks.

Références

  • Virlogeux A et al (2018) Reconstituting Corticostriatal Network On-a-Chip Reveals the Contribution of the Presynaptic Compartment to Huntington’s Disease. Cell Reports, Jan 2;22(1):110-122. doi: 10.1016/j.celrep.2017.12.013
  • Hinckelmann MV, et al., (2016) Self-propelling vesicles define glycolysis as the minimal energy machinery for neuronal transport Nature Communications, 7:13233. doi: 10.1038/ncomms13233
  • Zala D, et al. (2013) Vesicular glycolysis provides on-board energy for axonal transport. Cell. 152, 479-91. doi: 10.1016/j.cell.2012.12.029

Domaines d'expertise requis

Cell biology, neurobiology, imaging techniques, microscopy, microfluidics

Contacts

Frédéric Saudou, UGA Professor, and Chiara Scaramuzzino, Researcher
Email : frederic.saudou@univ-grenoble-alpes.fr

Mise à jour le 31 mai 2018

Contacts

Pour une candidature spontanée, envoyez un email à gincomm[at]univ-grenoble-alpes.fr ou utilisez le formulaire de contact.

Pour les stages de licence et de 3ème, voir les modalités de demande spécifiques sur les pages correspondantes. 

Membres
Associés renforcés
Associés simples