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Grenoble Institut des Neurosciences Grenoble Institut des Neurosciences

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Characterization of a novel animal model of Parkinson’s disease

Objectifs

The objectives of this project are to develop and to characterize a new mouse model of Parkinson’s disease that will represent an important step towards a better understanding of the pathogenic mechanisms and the discovery of novel therapies.

Résumé

To date, modelling Parkinson’s disease (PD)-like phenotype in rodents requires the use of toxic substances or excellent skills in stereotaxic surgery. These methods go along with variabilities in the results which limit the attractiveness of these models. In parallel, transgenic mouse models of PD only mimic pre-symptomatic forms of the disease. Hence, it is crucial to develop novel models of PD that are relevant, reproducible, and that elicit behavioural and cellular phenotypes reminiscent to the human pathology. We will take advantage of a novel viral vector allowing, via a simple systemic injection, to express a transgene throughout the central nervous system. We will combine this new tool with a genetic construct expressing human alpha-synuclein under the control of a dopamine neuron-specific promoter to induce a Parkinson-like phenotype. Behavioural tests will be used to assess locomotor and non-motor symptoms and the neuronal pathology will be studied with a broad range of histological, biochemical and molecular techniques.

Méthodes

The student will learn how to produce plasmids and adeno-associated viral vectors, and to perform intra-venous injections in mice. He/She will be trained to carry out various behavioural tests. Post-mortem studies will include histological analysis, confocal microscopy and western blotting.

Références

  • Tamburrino et al., (2015) Acta Neuropathologica Communcations; PMID: 26666562
  • Decressac et al., (2012) Science Translational Medicine; PMID: 23220632
  • Decressac et al., (2012) Experimental Neurology; PMID: 22394547

Domaines d'expertise requis

Alpha-synuclein, viral vectors, behavioural tests, histology, western blotting

Contacts

Dr. M.Decressac - Group leader
Email : michael.decressac@univ-grenoble-alpes.fr
Tél: 04 56 52 06 75

Mise à jour le 6 juin 2019

Contacts

Pour les stages (master, licence, 3ème), envoyer directement un email au responsable de l'équipe que vous avez identifiée.

Pour une candidature spontanée pour un emploi et uniquement pour cela, envoyez un email à gincomm[at]univ-grenoble-alpes.fr ou utilisez le formulaire de contact.

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