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Grenoble Institut des Neurosciences Grenoble Institut des Neurosciences

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Easier, deeper, healthier, sharper – major advances in STED and MINFLUX microscopies

on the June 10, 2021
11:30

Seminar by Frédéric Eghiaian (Abberior GmbH, Göttingen)

By increasing the separative power of fluorescence techniques by a 2-to-10-fold factor, super-resolution techniques help improve our understanding of many biological processes in vitro or in cells. However, diffraction-unlimited techniques (localization microscopies & STED) have for a long time been subjected to practical limitations related to high illumination powers, phototoxicity and incompatibility with deep samples among others. In this presentation, drastic methodological improvements in STED microscopy will be shown. Adaptative illumination techniques such as RESCUE and DyMIN now enable 3D-STED down to 75nm resolution with reduced photobleaching, as well as STED observations on living cells. Adaptative optics is a new promising avenue for STED and confocal observations in tissues, as well as MATRIX detector for noise suppression. Combined with development of new dyes and improved usability, those new methods considerably broaden the scope of STED. Finally, MINFLUX – a novel localization and tracking method with nanometer resolution - will be described.

Hosts : Yasmina Saoudi and Frédéric Saudou.

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Amphi Kampf and / or Zoom link, subject to prevailing Covid-related restrictions
Updated on April 19, 2021

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