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Grenoble Institut des Neurosciences Grenoble Institut des Neurosciences

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Truncation of tau and cytoskeleton alteration in neurons

Objectives

Truncated forms of tau are present in neurons during brain disorders like Alzheimer’s disease. These truncated proteins may exhibit toxic gain of functions, leading to cytoskeleton changes and neuronal impairments. The main objective is to investigate how tau fragments affect neuronal cytoskeleton properties.
 

Abstract

Tau is a cytoskeleton-associated protein involved in microtubule and actin regulation, which contributes to the proper functions of neurons. Since the identification of tau as a major component of the neurofibrillary tangles characterizing Alzheimer’s disease, much attention has been given to tau aggregative properties. However, recent studies have proposed that soluble truncated forms of tau exhibit toxic gain of functions, leading to cytoskeleton changes and neuronal impairments. Yet, the mechanisms by which these truncated forms alter microtubule and actin networks are still unknown. The student will explore the cytoskeleton- regulative properties of tau fragments in cultured primary hippocampal neurons transduced with each fragment by the mean of lentiviral vectors, already available in the laboratory. Using expansion protocols and super-resolution microscopy, the student will determine the effects of tau fragments on microtubule and actin arrays present in neuronal processes.
 

Methods

Mouse brain dissection, cell culture (primary hippocampal neurons), transduction with lentiviral vectors, expansion methods, immunofluorescence, Airyscan confocal microscopy.
 

References


Requested domains of expertise

Cellular and molecular biology, optical microscopy, English.

Contacts

Anne Fourest-Lieuvin, PhD, Researcher, CEA
Email : anne.fourest-lieuvin@univ-grenoble-alpes.fr
Phone : 33 (0)4 56 52 06 89
 

Updated on June 24, 2021

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