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Grenoble Institut des Neurosciences Grenoble Institut des Neurosciences

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MAP6 and neuronal cytoskeletons

Objectives

Deciphering the intimate relation of MAP6 protein with the different cytoskeletons present in neurons (actin and microtubule) using expansion and high-resolution microscopy
 

Abstract

Microtubules are hollow cylinders composed of heterodimer of alpha- and beta- tubulins. Tubulin dimers assemble to form protofilaments which then associates to build a microtubule.
MAP6 is a structural MAP (Microtubule Associated Protein) able to protect microtubules against cold- induced depolymerization. We had recently shown that MAP6, on top of binding to the external surface of the microtubule, generates highly stable microtubules by localizing inside the microtubule lumen. MAP6 is thus the first neuronal microtubule inner protein (MIP) identified so far.
We propose to use expansion microscopy, a technic that increases the size of objects crosslinked into acrylamide gels, in association with Super Resolution microscopy, to reach the resolution allowing the visualization of individual microtubules in neurons. We will be thus able to localize microtubules associated with MAP6 within the whole population of neuronal microtubules.
 

Methods

Neuronal culture; Immunofluorescence; Expansion microscopy; Confocal microscopy; High resolution microscopy (Airyscan and SR-Spinning disk)
 

References

 

Requested domains of expertise

Cell biology; Microscopy; Images analysis
 

Contacts

Sylvie Gory-Fauré, Researcher, Inserm
Emails : sylvie.gory-faure@univ-grenoble-alpes.fr

 

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Updated on June 29, 2021

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