Deciphering the role of the translational complex and protein synthesis in Central Nervous System regeneration
Objectives
Our lab seeks is interested in axon regeneration mechanisms. Our project uses in vivo and in vitro systems to decipher processes controlling axonal growth in adult in order to rebuild circuit after traumatic or chronic injury of the central nervous system.
Abstract
Unlike the peripheral nervous system, neurons from the central nervous system (brain, spinal cord) are not able to regrow axons after injury. Therefore, any lesion to the CNS leads to permanent motor and/or cognitive impairment. To this day, no treatment is available to overcome CNS injuries. It is now well established that modulating neurons themselves promotes axonal growth. The challenge now is to identify neuronal targets and molecular pathways that are required for axon regeneration. Our lab is focus on the analysis of the injury signal in order to define new way to promote axon regeneration. We are using a combination of in-vitro, in-vivo assay, molecular biology and biochemistry to characterized new cellular pathway implicated in neuroprotection and axonal growth in order to develop new therapeutic strategies.
Methods
Methods developed in the team are: in-vivo model of central nervous system injury, western-blot analysis, immunofluorescence. Biochemistry technics link to the analysis of ribosome and ribosomal proteins.
References
Proteomics analysis reveal modifications in brain visual targets following optic nerve injury. Noémie Vilallongue*, Julia Schaeffer*, Anne Marie Hesse, Céline Delpech, Yohan Couté, Stephane Belin+, Homaira Nawabi+. *equal contribution, +co-corresponding BIORXIV doi: https://doi.org/10.1101/2021.03.26.437129
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